Figure 1a: Bacterial growth with DNA plasmid added. Plate with ampicillin. [02-27-14, SOC media, BL21 (DE3) bacteria, pGEM, gbr22 DNA plasmid]
Figure 1a: Bacterial growth with DNA plasmid added. Plate with ampicillin. [02-27-14, SOC media, BL21 (DE3) bacteria, pGEM, gbr22 DNA plasmid]
Figure 1b: Bacterial growth with no DNA plasmid added. [02-27-14, SOC media, BL21 (DE3) bacteria, pGEM, no DNA]
Figure 1c: Fun plate with bacteria from a sink sponge. No ampicillin.
Figure 1d: Another group’s bacteria with DNA. This the sample used for growing the starter culture. [02-27-14, BL21(DE3) SOC media, pGEM]
Figure 2a:Sample 1 after letting the new bacteria cells recover and grow. [02-28-14, BL21(DE3), pGEM, gbr22 DNA, ampicillin, LB broth] Figure 2b:Sample 2 after letting the new bacteria cells recover and grow. [02-28-14, BL21(DE3), pGEM, gbr22 DNA, ampicillin, LB broth] Figure 3a:Sample #1 after centrifuging and disposing of the waste; new bacterial cells present in purple. Figure 3b:Sample 2 after centrifuging and disposing of the waste; new bacterial cells present in purple.
Figure 4:
Sample after purification. The purple is Elution 1. The white is Elution 2.
Figure 5:
Samples 1-6 flow throughs, stained on the gel. Gel is dried and labeled.
Introduction:
Materials & Methods:
Results:
Figure 1a: Bacterial growth with DNA plasmid added. Plate with ampicillin. [02-27-14, SOC media, BL21 (DE3) bacteria, pGEM, gbr22 DNA plasmid]
Figure 1b: Bacterial growth with no DNA plasmid added. [02-27-14, SOC media, BL21 (DE3) bacteria, pGEM, no DNA]
Figure 1c: Fun plate with bacteria from a sink sponge. No ampicillin.
Figure 1d: Another group’s bacteria with DNA. This the sample used for growing the starter culture. [02-27-14, BL21(DE3) SOC media, pGEM]
Figure 2a:Sample 1 after letting the new bacteria cells recover and grow. [02-28-14, BL21(DE3), pGEM, gbr22 DNA, ampicillin, LB broth]
Figure 2b:Sample 2 after letting the new bacteria cells recover and grow. [02-28-14, BL21(DE3), pGEM, gbr22 DNA, ampicillin, LB broth]
Figure 3a:Sample #1 after centrifuging and disposing of the waste; new bacterial cells present in purple.
Figure 4:
Sample after purification. The purple is Elution 1. The white is Elution 2.
Figure 5:
Samples 1-6 flow throughs, stained on the gel. Gel is dried and labeled.
Discussion:
Conclusions:
References: